What is an Autoclave? Office of Environmental Health and Safety

Autoclaves use water, pressure, and heat to create superheated steam that kills microorganisms and spores. Autoclaves at Princeton are used to decontaminate certain biological waste and sterilize media, instruments and lab ware.  Superheated Steam How's That Work?  Steam sterilization is mainly a function of temperature, pressure and time:  T

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