Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

Scientific Article | Ce protocole décrit en détail la préparation des complexes nucléosomiques à l’aide de deux méthodes de préparation des échantillons
DNA repair in the context of chromatin is poorly understood. Biochemical studies using nucleosome core particles, the fundamental repeating unit of chromatin, show most DNA repair enzymes remove DNA damage at reduced rates as compared to free DNA.

Cryo–electron microscopy structure of the H3-H4 octasome: A

High-resolution structure determination of sub-100 kDa complexes

JoVE Peer Reviewed Scientific Video Journal - Methods and Protocols

The cryo-electron microscopic structure of the ELYSC–nucleosome

Preparation of Nucleosome Core Particles Complexed with DNA Repair

Cryo–electron microscopy structure of the H3-H4 octasome: A

Structural basis of human transcription–DNA repair coupling

Structural plasticity of the nucleosome core particle (NCP). a

Nucleosome core particle dimers. (A) Representative cryo-EM raw

Self-Assembly of Geometry-Based DNA Origami-Histone Protein Hybrid

Nucleosome Structure and Function