Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
![Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination](https://cloudfront.jove.com/files/ftp_upload/64061/64061fig02.jpg)
Scientific Article | Ce protocole décrit en détail la préparation des complexes nucléosomiques à l’aide de deux méthodes de préparation des échantillons
DNA repair in the context of chromatin is poorly understood. Biochemical studies using nucleosome core particles, the fundamental repeating unit of chromatin, show most DNA repair enzymes remove DNA damage at reduced rates as compared to free DNA.
![](https://www.pnas.org/cms/10.1073/pnas.2206542119/asset/cc3fa443-76c1-4c11-9fcc-9e6efbcb7474/assets/images/large/pnas.2206542119fig04.jpg)
Cryo–electron microscopy structure of the H3-H4 octasome: A
![](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41467-019-08991-8/MediaObjects/41467_2019_8991_Fig1_HTML.png)
High-resolution structure determination of sub-100 kDa complexes
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JoVE Peer Reviewed Scientific Video Journal - Methods and Protocols
![](https://www.researchgate.net/publication/332842351/figure/fig3/AS:959989103464454@1605890646236/The-cryo-electron-microscopic-structure-of-the-ELYSC-nucleosome-complex-a-Digital.png)
The cryo-electron microscopic structure of the ELYSC–nucleosome
![](https://cloudfront.jove.com/files/ftp_upload/64061/64061fig01.jpg)
Preparation of Nucleosome Core Particles Complexed with DNA Repair
![](https://www.pnas.org/cms/10.1073/pnas.2206542119/asset/b5f829bf-dbad-4ad7-b375-43f03bac0be1/assets/images/large/pnas.2206542119fig05.jpg)
Cryo–electron microscopy structure of the H3-H4 octasome: A
![](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41586-021-03906-4/MediaObjects/41586_2021_3906_Fig1_HTML.png)
Structural basis of human transcription–DNA repair coupling
![](https://www.researchgate.net/publication/324261494/figure/fig1/AS:958860017803268@1605621451338/Structural-plasticity-of-the-nucleosome-core-particle-NCP-a-Cryo-EM-maps-of-the-NCP-in.jpg)
Structural plasticity of the nucleosome core particle (NCP). a
![](https://www.researchgate.net/publication/324951773/figure/fig1/AS:622610815672321@1525453394653/Nucleosome-core-particle-dimers-A-Representative-cryo-EM-raw-micrographs-collected-on.png)
Nucleosome core particle dimers. (A) Representative cryo-EM raw
Self-Assembly of Geometry-Based DNA Origami-Histone Protein Hybrid
![](https://pubs.acs.org/cms/10.1021/cr500373h/asset/images/medium/cr-2014-00373h_0015.gif)
Nucleosome Structure and Function